Jump to main content
Jump to site search

Issue 5, 2014
Previous Article Next Article

A new colorimetric strategy for monitoring caspase 3 activity by HRP-mimicking DNAzyme–peptide conjugates

Author affiliations

Abstract

A new method for caspase 3 activity assay has been developed based on HRP-mimicking DNAzyme–peptide conjugates. The mechanism of detection was based on the specific cleavage of DEVD-peptides by active caspase 3 for recognition and the catalytic properties of HRP-mimicking DNAzymes for signal amplification. Under optimal conditions, the detection limit of caspase 3 was 0.89 nM. The proposed method was also successfully applied for the detection of caspase 3 in apoptosis cell lysates.

Graphical abstract: A new colorimetric strategy for monitoring caspase 3 activity by HRP-mimicking DNAzyme–peptide conjugates

Back to tab navigation

Supplementary files

Article information


Submitted
28 Oct 2013
Accepted
14 Dec 2013
First published
17 Dec 2013

Analyst, 2014,139, 1178-1183
Article type
Paper
Author version available

A new colorimetric strategy for monitoring caspase 3 activity by HRP-mimicking DNAzyme–peptide conjugates

Z. Zhou, L. Peng, X. Wang, Y. Xiang and A. Tong, Analyst, 2014, 139, 1178
DOI: 10.1039/C3AN02028B

Social activity

Search articles by author

Spotlight

Advertisements