Issue 37, 2013
From the journal:

Journal of Materials Chemistry B

Self-assembly of suspended collagen films and their viability as cell culture substrates†

Megan J. Roberts,ab   Niharika Bhatt,c   Chris M. Voge,‡c   Eric R. Meshot,§a   Jan P. Stegemannc  and  A. John Hart*ab  

* Corresponding authors

a Department of Mechanical Engineering, University of Michigan, Ann Arbor, MI 48109, USA

b Department of Mechanical Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
E-mail: ajhart@mit.edu

c Department of Biomedical Engineering, University of Michigan, Ann Arbor, MI 48109, USA
E-mail: jpsteg@umich.edu

Abstract

We present the fabrication and mechanical properties of thin collagen networks self-assembled in a suspended configuration over micropost arrays. These collagen “canopies” were formed on arrays of microposts made of PDMS, silicon, and vertically aligned carbon nanotubes (CNT). We reversibly loaded the canopy to an in-plane stress of 32 MPa. We found that human dermal fibroblasts (HDFb) proliferate on the canopy substrates for up to 7 days. This versatile fabrication method for suspended extracellular matrix (ECM) films may enable the development of new assays to probe cell–ECM interactions, along with integration of microelectronic probes.

Graphical abstract: Self-assembly of suspended collagen films and their viability as cell culture substrates

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Article information

DOI
https://doi.org/10.1039/C3TB20800A
Article type
Communication
Submitted
05 Jun 2013
Accepted
12 Aug 2013
First published
12 Aug 2013

J. Mater. Chem. B, 2013,1, 4711-4718

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Self-assembly of suspended collagen films and their viability as cell culture substrates

M. J. Roberts, N. Bhatt, C. M. Voge, E. R. Meshot, J. P. Stegemann and A. J. Hart, J. Mater. Chem. B, 2013, 1, 4711 DOI: 10.1039/C3TB20800A

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