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Issue 36, 2013
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Potent calcium phosphate nanoparticle surface coating for in vitro and in vivo siRNA delivery: a step toward multifunctional nanovectors

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Abstract

The present study describes hybrid nanoparticles, built by alternate deposition of siRNA and modified polyethyleneimine (tyrosine-grafted PEI or tyrosine/galactose-grafted PEI) on calcium phosphate nanoparticles. These “easy to produce” nanoparticles (NPs) present an efficient gene silencing effect demonstrated in vitro in a luciferase expressing cell culture model and in vivo in a tumour xenograft mouse model. The luciferase gene silencing percentage reached up to 95% in vitro with biocompatible doses of siRNA. Interestingly, we show by SPECT imaging of radiolabeled particles that without modifying the size, stability and in vitro efficiency, the grafting of a sugar moiety on PEI can modify the in vivo biodistribution of the particles. The proof of concept that galactose-grafting on PEI could change biodistribution without changing the gene silencing efficiency makes them versatile tools for specific delivery of small interfering RNA. As they have been designed so far, biodistribution is mainly located in the liver and thus these innovative nanoparticles open a realistic and feasible strategy for siRNA delivery into the liver in vivo.

Graphical abstract: Potent calcium phosphate nanoparticle surface coating for in vitro and in vivo siRNA delivery: a step toward multifunctional nanovectors

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Publication details

The article was received on 18 Apr 2013, accepted on 05 Jul 2013 and first published on 08 Jul 2013


Article type: Paper
DOI: 10.1039/C3TB20557F
J. Mater. Chem. B, 2013,1, 4692-4700

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    Potent calcium phosphate nanoparticle surface coating for in vitro and in vivo siRNA delivery: a step toward multifunctional nanovectors

    T. Devarasu, R. Saad, A. Ouadi, B. Frisch, E. Robinet, P. Laquerrière, J. Voegel, T. Baumert, J. Ogier and F. Meyer, J. Mater. Chem. B, 2013, 1, 4692
    DOI: 10.1039/C3TB20557F

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