Jump to main content
Jump to site search

Issue 27, 2013
Previous Article Next Article

Growth and spontaneous differentiation of umbilical-cord stromal stem cells on activated carbon cloth

Author affiliations

Abstract

We have investigated the capacity of activated carbon cloth to support the growth and differentiation of human mesenchymal umbilical-cord stromal stem cells. Our results demonstrate that this scaffold provides suitable conditions for the development of cell-derived matrix proteins and facilitates the growth of undifferentiated stem cells with the ability to induce osteogenic and chondrogenic differentiation. Immunoflourescence staining revealed extensive expression of collagen in all the samples, and collagen type II and osteopontin within the samples cultivated in specific differentiation-inducing media. Cell growth and the formation of natural collagen, calcium–magnesium carbonate and hydroxyapatite crystals, together with the self-assemblage of collagen to produce suprafibrillar arrangements of fibrils all occur simultaneously and can be studied together ex vivo under physiological conditions. Furthermore, the spontaneous differentiation of stem cells cultured on activated carbon cloth with no osteogenic supplements opens up new possibilities for bone-tumour engineering and treatment of traumatic and degenerative bone diseases.

Graphical abstract: Growth and spontaneous differentiation of umbilical-cord stromal stem cells on activated carbon cloth

Back to tab navigation

Supplementary files

Publication details

The article was received on 05 Mar 2013, accepted on 23 May 2013 and first published on 23 May 2013


Article type: Paper
DOI: 10.1039/C3TB20305K
Citation: J. Mater. Chem. B, 2013,1, 3359-3368

  •   Request permissions

    Growth and spontaneous differentiation of umbilical-cord stromal stem cells on activated carbon cloth

    V. de Araújo Farias, J. J. López-Peñalver, J. Sirés-Campos, M. V. López-Ramón, C. Moreno-Castilla, F. J. Oliver and J. M. Ruiz de Almodóvar, J. Mater. Chem. B, 2013, 1, 3359
    DOI: 10.1039/C3TB20305K

Search articles by author

Spotlight

Advertisements