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Issue 22, 2013
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Synthesis, stability, cellular uptake, and blood circulation time of carboxymethyl-inulin coated magnetic nanoparticles

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Abstract

Iron oxide nanoparticles were coated with the biocompatible, biodegradable, non-immunogenic polysaccharide inulin by introduction of carboxyl groups into the inulin structure and conjugation with amine groups on the surface of iron oxide nanoparticles grafted with 3-aminopropyltriethoxysilane. The resulting nanoparticles were characterized by FT-IR spectroscopy, transmission electron microscopy, dynamic light scattering, zeta potential, SQUID magnetometry, and with respect to their energy dissipation rate in applied alternating magnetic fields. The nanoparticles had a hydrodynamic diameter in the range of 70 ± 10 nm and were superparamagnetic, with energy dissipation rates in the range of 58–175 W g−1 for an applied field frequency of 233 kHz and an applied field amplitude in the range of 20–48 kA m−1. The nanoparticles were stable in a range of pH, at temperatures between 23 °C and 53 °C, and in short term storage in water, PBS, and culture media. The particles were non-cytotoxic to the immortalized human cancer cell lines Hey A8 FDR, A2780, MDA 468, MCF-7 and Caco-2. The nanoparticles were readily taken up by Caco-2 cells in a time and concentration dependent fashion, and were found to have a pharmacokinetic time constant of 47 ± 3 min. The small size, non-cytotoxicity, and efficient energy dissipation of the particles could make them useful for biomedical applications such as magnetic fluid hyperthermia.

Graphical abstract: Synthesis, stability, cellular uptake, and blood circulation time of carboxymethyl-inulin coated magnetic nanoparticles

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Publication details

The article was received on 20 Feb 2013, accepted on 15 Apr 2013 and first published on 15 Apr 2013


Article type: Paper
DOI: 10.1039/C3TB20256A
Citation: J. Mater. Chem. B, 2013,1, 2807-2817

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    Synthesis, stability, cellular uptake, and blood circulation time of carboxymethyl-inulin coated magnetic nanoparticles

    L. Santiago-Rodríguez, M. M. Lafontaine, C. Castro, J. Méndez-Vega, M. Latorre-Esteves, E. J. Juan, E. Mora, M. Torres-Lugo and C. Rinaldi, J. Mater. Chem. B, 2013, 1, 2807
    DOI: 10.1039/C3TB20256A

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