Issue 8, 2013

Controlling performance of lipase immobilised on bioinspired silica

Abstract

Lipase (Candida antarctica lipase B) was immobilised on silica that was produced via a mild route using a recently developed biologically inspired silica formation method. This route of immobilisation was favourable compared to traditional methods due to its simplicity, mild conditions, low cost, one-step procedure and short preparation time. Lipase was chosen as the enzyme due to its wide implementation in industry, particularly in the food and pharmaceutical industries. Compared to other methods using bioinspired silica entrapment of enzymes, we demonstrate several significant improvements in the performance of the biocatalysts produced. Very high immobilisation efficiencies (close to 100%) were achieved under optimised conditions. The immobilised enzymes also displayed high levels of activity, which exceeded those reported in previous studies of lipase that was immobilised on bioinspired silica. Furthermore, we demonstrate the control over enzyme activity which was achieved through the entrapment conditions by regulating surface area, the average pore diameter and the mean particle size of the silica support. The immobilised enzymes also had very good reuse potential, and showed improved thermal and pH stability. The biocatalysts also performed well in turbulent conditions, showed good storage properties and were successfully used in bench-top reactors, therefore strongly supporting their use in commercial applications. The immobilised enzymes also performed comparably to or better than the industrial benchmark, Novozym® 435. The bioinspired method of immobilisation used has significant benefits over current methods, and these factors could increase potential applications in industry.

Graphical abstract: Controlling performance of lipase immobilised on bioinspired silica

Supplementary files

Article information

Article type
Paper
Submitted
27 Aug 2012
Accepted
21 Dec 2012
First published
09 Jan 2013

J. Mater. Chem. B, 2013,1, 1164-1174

Controlling performance of lipase immobilised on bioinspired silica

C. Forsyth and S. V. Patwardhan, J. Mater. Chem. B, 2013, 1, 1164 DOI: 10.1039/C2TB00462C

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements