Issue 22, 2013
From the journal:

Soft Matter

Solvent effects on protein fast dynamics: implications for biopreservation†

Victoria García Sakai,*a   Sheila Khodadadi,‡b   Marcus T. Cicerone,c   Joseph E. Curtis,b   Alexei P. Sokolovde  and  Joon Ho Roh§*f  

* Corresponding authors

a ISIS Neutron and Muon Facility, Rutherford Appleton Laboratory, Science & Technology Facilities Council, Didcot, UK
E-mail: Victoria.garcia-sakai@stfc.ac.uk

b NIST Center for Neutron Research, National Institute of Standards and Technology, Gaithersburg, MD 20899, USA
E-mail: joseph.curtis@nist.gov

c Polymers Division, National Institute of Standards and Technology, Gaithersburg, MD 20899, USA
E-mail: cicerone@nist.gov

d Chemical Sciences Division, Oak Ridge National Laboratory, Oak Ridge, TN 37831, USA

e Dept. of Chemistry, University of Tennessee, Knoxville, TN 37996, USA
E-mail: sokolov@utk.edu

f Polymer Science, University of Akron, Akron, OH 44325, USA
E-mail: joonho.roh.ctr.ks@nrl.navy.mil

Abstract

In the context of biopreservation, we study the influence of water, glycerol and trehalose on the ps–ns dynamics of lyzosyme using neutron scattering. Results indicate that the choice of bioprotectant depends on the storage temperature; glycerol is the most effective for low temperatures and trehalose for high temperatures.

Graphical abstract: Solvent effects on protein fast dynamics: implications for biopreservation

About
Cited by
Related
Download options Please wait...

Supplementary files

Article information

DOI
https://doi.org/10.1039/C3SM50492A
Article type
Communication
Submitted
17 Feb 2013
Accepted
08 Apr 2013
First published
29 Apr 2013

Soft Matter, 2013,9, 5336-5340

Permissions

Request permissions

Solvent effects on protein fast dynamics: implications for biopreservation

V. G. Sakai, S. Khodadadi, M. T. Cicerone, J. E. Curtis, A. P. Sokolov and J. H. Roh, Soft Matter, 2013, 9, 5336 DOI: 10.1039/C3SM50492A

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements