Issue 29, 2013
From the journal:

Soft Matter

Site-specific, covalent incorporation of Tus, a DNA-binding protein, on ionic-complementary self-assembling peptide hydrogels using transpeptidase Sortase A as a conjugation tool†‡

Susanna Piluso,a   Heather C. Cassell,b   Jonathan L. Gibbons,c   Thomas E. Waller,a   Nick J. Plant,b   Aline F. Millerc  and  Gabriel Cavalli*a  

* Corresponding authors

a Department of Chemistry, University of Surrey, Guildford, UK
E-mail: g.cavalli@surrey.ac.uk
Tel: +44 (0)1483 686837

b Department of Biochemistry and Physiology, University of Surrey, Guildford, UK
E-mail: n.plant@surrey.ac.uk
Tel: +44 (0)1483 686412

c Manchester Institute of Biotechnology, School of Chemical Engineering & Analytical Science, University of Manchester, 131 Princess Street, Manchester, UK
E-mail: aline.miller@manchester.ac.uk
Tel: +44 (0)161 3065781

Abstract

The site-specific conjugation of DNA-binding protein (Tus) to self-assembling peptide FEFEFKFKK was demonstrated. Rheology studies and TEM of the corresponding hydrogels (including PNIPAAm-containing systems) showed no significant variation in properties and hydrogel morphology compared to FEFEFKFKK. Critically, we demonstrate that Tus is accessible within the gel network displaying DNA-binding properties.

Graphical abstract: Site-specific, covalent incorporation of Tus, a DNA-binding protein, on ionic-complementary self-assembling peptide hydrogels using transpeptidase Sortase A as a conjugation tool

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Article information

DOI
https://doi.org/10.1039/C3SM00131H
Article type
Communication
Submitted
11 Jan 2013
Accepted
27 Feb 2013
First published
07 Mar 2013
This article is Open Access

Soft Matter, 2013,9, 6752-6756

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Site-specific, covalent incorporation of Tus, a DNA-binding protein, on ionic-complementary self-assembling peptide hydrogels using transpeptidase Sortase A as a conjugation tool

S. Piluso, H. C. Cassell, J. L. Gibbons, T. E. Waller, N. J. Plant, A. F. Miller and G. Cavalli, Soft Matter, 2013, 9, 6752 DOI: 10.1039/C3SM00131H

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