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Issue 9, 2013
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A novel approach to the site-selective dual labelling of a proteinvia chemoselective cysteine modification

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Abstract

Local protein microenvironment is used to control the outcome of reaction between cysteine residues and 2,5-dibromohexanediamide. The differential reactivity is exploited to introduce two orthogonal reactive handles onto the surface of a double cysteine mutant of superfolder green fluorescent protein in a regioselective manner. Subsequent elaboration with commonly used thiol and alkyne containing reagents affects site-selective protein dual labelling.

Graphical abstract: A novel approach to the site-selective dual labelling of a protein via chemoselective cysteine modification

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Article information


Submitted
13 May 2013
Accepted
17 Jun 2013
First published
18 Jun 2013

This article is Open Access
All publication charges for this article have been paid for by the Royal Society of Chemistry

Chem. Sci., 2013,4, 3455-3458
Article type
Edge Article

A novel approach to the site-selective dual labelling of a protein via chemoselective cysteine modification

R. I. Nathani, P. Moody, V. Chudasama, M. E. B. Smith, R. J. Fitzmaurice and S. Caddick, Chem. Sci., 2013, 4, 3455
DOI: 10.1039/C3SC51333E

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