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Issue 10, 2013
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Electrocatalytically active multi-protein assemblies using nanoscaled building blocks

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Abstract

Biosensors based on nanomaterials constitute an emerging area of interdisciplinary research. In particular in electrochemical sensors, electron transfer cascades can be used for defined signal generation. Our study describes the investigation of silica nanoparticles (SiNPs), DNA, cytochrome c (cyt c) and cellobiose dehydrogenase (CDH) for the development of catalytically active multi-protein assemblies. We report on direct and interprotein electron transfer reaction cascades of CDH and cyt c in an immobilized form by means of nanoscaled building blocks: Carboxy-modified SiNPs, and DNA. The building blocks provide an artificial matrix, which permit protein arrangement in an electro- and catalytically-active form. Multilayered protein architectures on electrodes featuring direct and interprotein electron transfer by the use of entirely different nanoscaled building blocks has been established for the first time. In addition we highlight, that the secondary building blocks (DNA or SiNPs) used for the construction as well as the glycosylation of the enzyme (CDH) play a key role for the mode of operation in such complex entities.

Graphical abstract: Electrocatalytically active multi-protein assemblies using nanoscaled building blocks

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Publication details

The article was received on 08 Nov 2012, accepted on 07 Jan 2013 and first published on 08 Jan 2013


Article type: Paper
DOI: 10.1039/C2RA22819J
Citation: RSC Adv., 2013,3, 3428-3437
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    Electrocatalytically active multi-protein assemblies using nanoscaled building blocks

    S. C. Feifel, A. Kapp, R. Ludwig, L. Gorton and F. Lisdat, RSC Adv., 2013, 3, 3428
    DOI: 10.1039/C2RA22819J

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