Issue 44, 2013
From the journal:

RSC Advances

Enzymatic synthesis of colorimetric substrates to determine α-2,3- and α-2,6-specific neuraminidase activity†

Juana Elizabeth Reyes Martínez,a   Robert Šardzík,a   Josef Voglmeirb  and  Sabine L. Flitsch*a  

* Corresponding authors

a MIB & School of Chemistry, The University of Manchester, 131 Princess Street, Manchester, UK
E-mail: sabine.flitsch@manchester.ac.uk
Fax: +44 (0)161 2751311
Tel: +44 (0)161 3065172

b Glycomics and Glycan Bioengineering Research Center, Nanjing Agricultural University, China

Abstract

Glycoconjugates containing either terminal α-2,3- or α-2,6-Neu5Ac-Gal disaccharides are found on cell surfaces of many animal glycans. Each linkage can be specifically recognized by lectins and enzymes such as neuraminidases. Here we describe a one-step enzymatic synthesis of two colorimetric substrates that allow for fast distinction of specific neuraminidase activity.

Graphical abstract: Enzymatic synthesis of colorimetric substrates to determine α-2,3- and α-2,6-specific neuraminidase activity

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Article information

DOI
https://doi.org/10.1039/C3RA44791J
Article type
Communication
Submitted
30 Aug 2013
Accepted
11 Sep 2013
First published
27 Sep 2013

RSC Adv., 2013,3, 21335-21338

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Enzymatic synthesis of colorimetric substrates to determine α-2,3- and α-2,6-specific neuraminidase activity

J. E. Reyes Martínez, R. Šardzík, J. Voglmeir and S. L. Flitsch, RSC Adv., 2013, 3, 21335 DOI: 10.1039/C3RA44791J

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