Issue 37, 2013
From the journal:

RSC Advances

DNA display for drug discovery†

Haodong Chen,abc   Gangyi Chen,a   Feng Du,a   Qingquan Fu,a   Yun Zhaob  and  Zhuo Tang*a  

* Corresponding authors

a Natural Products Research Center, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, P. R. China
E-mail: tangzhuo@cib.ac.cn
Fax: +86 28 85243250
Tel: +86 28 85243250

b College of Life Sciences, Sichuan University, Chengdu 610064, P. R. China

c University of Chinese Academy of Sciences, Beijing 100049, P. R. China

Abstract

A novel DNA display strategy, based on a new puromycin modifier, has been developed. The 5′-end puromycin-tethered oligonucleotide was synthesized to hybridize with mRNA, so that it could attack the nascent polypeptides during in vitro translation. The DNA–peptide fusion molecule can tolerate more harsh and stringent selection conditions, therefore, this DNA display may become a useful tool for in vitro display technologies for the selection of peptide drug candidates.

Graphical abstract: DNA display for drug discovery

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Article information

DOI
https://doi.org/10.1039/C3RA42440E
Article type
Communication
Submitted
16 May 2013
Accepted
15 Jul 2013
First published
16 Jul 2013

RSC Adv., 2013,3, 16251-16254

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DNA display for drug discovery

H. Chen, G. Chen, F. Du, Q. Fu, Y. Zhao and Z. Tang, RSC Adv., 2013, 3, 16251 DOI: 10.1039/C3RA42440E

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