Issue 37, 2013

Ligase-assisted signal-amplifiable DNA detection using upconversion nanoparticles

Abstract

A novel ligase-assisted signal-amplifiable DNA detection scheme is demonstrated based on upconversion nanoparticles. Target DNA serves as a template for two designed DNA probes, one of which covalently attached to upconversion nanoparticles, to be joined into a single DNA strand by ligase. The resulting long DNA is able to form a hairpin loop structure, the number of which is amplified by thermal cycling. Energy transfer occurs between the upconversion nanoparticles and the intercalating dyes residing in the stem portion of the hairpin loop, and is monitored to quantify the amount of the initial target DNA. The method displays high sensitivity and specificity for DNA detection.

Graphical abstract: Ligase-assisted signal-amplifiable DNA detection using upconversion nanoparticles

Supplementary files

Article information

Article type
Communication
Submitted
15 May 2013
Accepted
08 Jul 2013
First published
09 Jul 2013

RSC Adv., 2013,3, 16326-16329

Ligase-assisted signal-amplifiable DNA detection using upconversion nanoparticles

P. Wang, T. O. Ahmadov, C. Lee and P. Zhang, RSC Adv., 2013, 3, 16326 DOI: 10.1039/C3RA42421A

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements