Issue 8, 2013

Synthesis of nanogel–protein conjugates


The covalent conjugation of bovine serum albumin (BSA) to disulfide cross-linked polymeric nanogels is reported. Polymeric nanogel precursors were synthesized via a reversible addition-fragmentation chain transfer (RAFT) random copolymerization of poly(ethylene glycol) methyl ether methacrylate (PEGMA) and pyridyl disulfide methacrylate (PDSMA). Reaction of the p(PEGMA-co-PDSMA) with dithiothreitol resulted in the formation of nanogels. PDSMA serves as both a crosslinking agent and a reactive handle for the surface modification of the nanogels. Lipophilic dye, DiI, was sequestered within the nanogels by performing the crosslinking reaction in the presence of the hydrophobic molecule. Thiol-enriched BSA was conjugated to nanogels loaded with DiI via a thiol-disulfide exchange reaction between the BSA and the surface exposed nanogel pyridyl disulfides. Conjugation was confirmed by fast protein liquid chromatography, dynamic light scattering, and agarose and polyacrylamide gel electrophoresis. We expect that this methodology is generally applicable to the preparation of nanogel–protein therapeutics.

Graphical abstract: Synthesis of nanogel–protein conjugates

Article information

Article type
18 Jan 2013
12 Feb 2013
First published
27 Feb 2013

Polym. Chem., 2013,4, 2464-2469

Synthesis of nanogel–protein conjugates

N. M. Matsumoto, D. C. González-Toro, R. T. Chacko, H. D. Maynard and S. Thayumanavan, Polym. Chem., 2013, 4, 2464 DOI: 10.1039/C3PY00085K

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