Issue 14, 2013
From the journal:

Organic & Biomolecular Chemistry

In vitro evolution of a Friedel–Crafts deoxyribozyme†

Utpal Mohan,a   Ritwik Buraia  and  Brian R. McNaughton*ab  

* Corresponding authors

a Department of Chemistry, Colorado State University, 200 West Lake Street, Fort Collins, Colorado, USA
E-mail: brian.mcnaughton@colostate.edu
Fax: (+1) 970-491-1801
Tel: (+1) 970-491-7060

b Department of Biochemistry and Molecular Biology, Colorado State University, 200 West Lake Street, Fort Collins, Colorado, USA

Abstract

We report the in vitro selection of a single-stranded 72-nucleotide DNA enzyme (deoxyribozyme) that catalyzes a Friedel–Crafts reaction between an indole and acyl imidazole in good yield and in aqueous solvent. Appreciable Friedel–Crafts product requires addition of copper nitrate and the deoxyribozyme. We observe deoxyribozyme-mediated bond formation for both in cis and in trans Friedel–Crafts reactions.

Graphical abstract: In vitro evolution of a Friedel–Crafts deoxyribozyme

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Article information

DOI
https://doi.org/10.1039/C3OB40080H
Article type
Communication
Submitted
15 Jan 2013
Accepted
15 Feb 2013
First published
18 Feb 2013

Org. Biomol. Chem., 2013,11, 2241-2244

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In vitro evolution of a Friedel–Crafts deoxyribozyme

U. Mohan, R. Burai and B. R. McNaughton, Org. Biomol. Chem., 2013, 11, 2241 DOI: 10.1039/C3OB40080H

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