Thiol–yne radical reaction mediated site-specific proteinlabelingvia genetic incorporation of an alkynyl-l-lysine analogue

Abstract

Three alkyne-containing pyrrolysine derivatives were synthesized and genetically encoded into proteins by a mutant PylRS–tRNA pair with high efficiencies. With these alkyne handles, site-specific dual labeling of proteins can be achieved via a bioorthogonal thiol–yne ligation reaction.