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Issue 16, 2013
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Copper-free click chemistry as an emerging tool for the programmed ligation of DNA-functionalised gold nanoparticles

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Abstract

We demonstrate a new method to program the ligation of single stranded DNA-modified gold nanoparticles using copper-free click chemistry. Gold nanoparticles functionalized with a discrete number of 3′-azide or 5′-alkyne modified oligonucleotides, can be brought together via a splint strand and covalently ‘clicked’, in a simple one-pot reaction. This new approach to the assembly of gold nanoparticles is inherently advantageous in comparison to the traditional enzymatic ligation. The chemical ligation is specific and takes place at room temperature by simply mixing the particles without the need for special enzymatic conditions. The yield of ‘clicked’ nanoparticles can be as high as 92%. The ease of the copper-free, ‘click-ligation’ method allows for its universal applicability and opens up new avenues in programmed nanoparticle organization.

Graphical abstract: Copper-free click chemistry as an emerging tool for the programmed ligation of DNA-functionalised gold nanoparticles

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Publication details

The article was received on 08 May 2013, accepted on 25 Jun 2013 and first published on 04 Jul 2013


Article type: Communication
DOI: 10.1039/C3NR02362A
Citation: Nanoscale, 2013,5, 7209-7212
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    Copper-free click chemistry as an emerging tool for the programmed ligation of DNA-functionalised gold nanoparticles

    A. Heuer-Jungemann, R. Kirkwood, A. H. El-Sagheer, T. Brown and A. G. Kanaras, Nanoscale, 2013, 5, 7209
    DOI: 10.1039/C3NR02362A

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