Issue 12, 2013
From the journal:

Nanoscale

Novel multistep BRET-FRET energy transfer using nanoconjugates of firefly proteins, quantum dots, and red fluorescent proteins†

Rabeka Alam,a   Joshua Zylstra,a   Danielle M. Fontaine,b   Bruce R. Branchinib  and  Mathew M. Maye*a  

* Corresponding authors

a Department of Chemistry, Syracuse University, Syracuse, New York, USA
E-mail: mmmaye@syr.edu

b Department of Chemistry, Connecticut College, New London, Connecticut, USA

Abstract

Sequential bioluminescence resonance energy transfer (BRET) and fluorescence resonance energy transfer (FRET) from firefly luciferase to red fluorescent proteins using quantum dot or rod acceptor/donor linkers is described. The effect of morphology and tuned optical properties on the efficiency of this unique BRET-FRET system was evaluated.

Graphical abstract: Novel multistep BRET-FRET energy transfer using nanoconjugates of firefly proteins, quantum dots, and red fluorescent proteins

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Article information

DOI
https://doi.org/10.1039/C3NR01842C
Article type
Communication
Submitted
12 Apr 2013
Accepted
29 Apr 2013
First published
20 May 2013

Nanoscale, 2013,5, 5303-5306

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Novel multistep BRET-FRET energy transfer using nanoconjugates of firefly proteins, quantum dots, and red fluorescent proteins

R. Alam, J. Zylstra, D. M. Fontaine, B. R. Branchini and M. M. Maye, Nanoscale, 2013, 5, 5303 DOI: 10.1039/C3NR01842C

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