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Issue 12, 2013
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Novel multistep BRET-FRET energy transfer using nanoconjugates of firefly proteins, quantum dots, and red fluorescent proteins

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Abstract

Sequential bioluminescence resonance energy transfer (BRET) and fluorescence resonance energy transfer (FRET) from firefly luciferase to red fluorescent proteins using quantum dot or rod acceptor/donor linkers is described. The effect of morphology and tuned optical properties on the efficiency of this unique BRET-FRET system was evaluated.

Graphical abstract: Novel multistep BRET-FRET energy transfer using nanoconjugates of firefly proteins, quantum dots, and red fluorescent proteins

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Publication details

The article was received on 12 Apr 2013, accepted on 29 Apr 2013 and first published on 20 May 2013


Article type: Communication
DOI: 10.1039/C3NR01842C
Nanoscale, 2013,5, 5303-5306

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    Novel multistep BRET-FRET energy transfer using nanoconjugates of firefly proteins, quantum dots, and red fluorescent proteins

    R. Alam, J. Zylstra, D. M. Fontaine, B. R. Branchini and M. M. Maye, Nanoscale, 2013, 5, 5303
    DOI: 10.1039/C3NR01842C

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