Visualizing the changes in the cellular redox environment using a novel profluorescent rhodamine nitroxide probe†
Abstract
A novel rhodamine nitroxide probe was synthesized for monitoring the cellular redox environment based on the reduction of the nitroxide to hydroxylamine. It displayed selective and sensitive response to biological reducing compounds. The different effects of anticancer agent 2-deoxyglucose on the redox environment of normal human liver cells and human hepatoma cells were studied by fluorescence imaging.