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Issue 23, 2013
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Ultrahigh-throughput sorting of microfluidic drops with flow cytometry

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Abstract

The detection and sorting of aqueous drops is central to microfluidic workflows for high-throughput biology applications, including directed evolution, digital PCR, and antibody screening. However, high-throughput detection and sorting of drops require optical systems and microfluidic components that are complex, difficult to build, and often yield inadequate sensitivity and throughput. Here, we demonstrate a general method to harness flow cytometry, with its unmatched speed and sensitivity, for droplet-based microfluidic sorting.

Graphical abstract: Ultrahigh-throughput sorting of microfluidic drops with flow cytometry

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Article information


Submitted
19 Jun 2013
Accepted
06 Oct 2013
First published
08 Oct 2013

Lab Chip, 2013,13, 4563-4572
Article type
Paper

Ultrahigh-throughput sorting of microfluidic drops with flow cytometry

S. W. Lim and A. R. Abate, Lab Chip, 2013, 13, 4563
DOI: 10.1039/C3LC50736J

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