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Issue 18, 2013
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Microfluidic titer plate for stratified 3D cell culture

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Abstract

Human tissues and organs are inherently heterogeneous. Their functionality is determined by the interplay between different cell types, their secondary architecture, vascular system and gradients of signaling molecules and metabolites. Here we propose a stratified 3D cell culture platform, in which adjacent lanes of gels and liquids are patterned by phaseguides to capture this tissue heterogeneity. We demonstrate 3D cell culture of HepG2 hepatocytes under continuous perfusion, a rifampicin toxicity assay and co-culture with fibroblasts. 4T1 breast cancer cells are used to demonstrate invasion and aggregation models. The platform is incorporated in a microtiter plate format that renders it fully compatible with automation and high-content screening equipment. The extended functionality, ease of handling and full compatibility to standard equipment is an important step towards adoption of Organ-on-a-Chip technology for screening in an industrial setting.

Graphical abstract: Microfluidic titer plate for stratified 3D cell culture

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Publication details

The article was received on 15 Feb 2013, accepted on 25 Jun 2013 and first published on 26 Jul 2013


Article type: Paper
DOI: 10.1039/C3LC50210D
Citation: Lab Chip, 2013,13, 3548-3554
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    Microfluidic titer plate for stratified 3D cell culture

    S. J. Trietsch, G. D. Israëls, J. Joore, T. Hankemeier and P. Vulto, Lab Chip, 2013, 13, 3548
    DOI: 10.1039/C3LC50210D

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