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Issue 8, 2013
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Lab-on-DVD: standard DVD drives as a novel laser scanning microscope for image based point of care diagnostics

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Abstract

We present a novel “Lab-on-DVD” system and demonstrate its capability for rapid and low-cost HIV diagnostics by counting CD4+ cells isolated from whole blood. We show that a commercial DVD drive can, with certain modifications, be turned into an improved DVD-based laser scanning microscope (DVD-LSM). The system consists of a multi-layered disposable polymer disc and a modified commercial DVD reader with rotational control for sample handling, temperature control for optimized bioassay, a photodiode array for detection, and software for signal processing and user interface – all the necessary components required for a truly integrated lab-on-a-chip system, with the capability to deliver high-resolution images down to 1 μm in size. Using discs modified with antibodies, we specifically captured CD4+ cells from whole blood, demonstrating single cell resolution imaging. The novel integrated DVD platform with sub-micron image resolution brings, for the first time, affordable cellular diagnostic testing to the point-of-care and should be readily applicable at resource-limited settings.

Graphical abstract: Lab-on-DVD: standard DVD drives as a novel laser scanning microscope for image based point of care diagnostics

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Article information


Submitted
11 Dec 2012
Accepted
01 Feb 2013
First published
04 Feb 2013

Lab Chip, 2013,13, 1578-1585
Article type
Paper

Lab-on-DVD: standard DVD drives as a novel laser scanning microscope for image based point of care diagnostics

H. Ramachandraiah, M. Amasia, J. Cole, P. Sheard, S. Pickhaver, C. Walker, V. Wirta, P. Lexow, R. Lione and A. Russom, Lab Chip, 2013, 13, 1578
DOI: 10.1039/C3LC41360H

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