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Issue 7, 2013
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Development of a single-cell array for large-scale DNA fluorescence in situ hybridization

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Abstract

DNA fluorescence in situ hybridization (FISH) is a powerful cytogenetic assay, but conventional sample-preparation methods for FISH do not support large-scale high-throughput data acquisition and analysis, which are potentially useful for several biomedical applications. To address this limitation, we have developed a novel FISH sample-preparation method based on generating a centimetre-sized cell array, in which all cells are precisely positioned and separated from their neighbours. This method is simple and capable of patterning nonadherent human cells. We have successfully performed DNA FISH on the single-cell arrays, which facilitates analysis of the FISH results with the FISH-FINDER computer program.

Graphical abstract: Development of a single-cell array for large-scale DNA fluorescence in situ hybridization

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Supplementary files

Article information


Submitted
17 Apr 2012
Accepted
30 Nov 2012
First published
04 Dec 2012

Lab Chip, 2013,13, 1316-1324
Article type
Paper

Development of a single-cell array for large-scale DNA fluorescence in situ hybridization

Y. Liu, B. Kirkland, J. Shirley, Z. Wang, P. Zhang, J. Stembridge, W. Wong, S. Takebayashi, D. M. Gilbert, S. Lenhert and J. Guan, Lab Chip, 2013, 13, 1316
DOI: 10.1039/C2LC40364A

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