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Issue 23, 2013
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Construction of a microfluidic chip, using dried-down reagents, for LATE-PCR amplification and detection of single-stranded DNA

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Abstract

LATE-PCR is an advanced form of non-symmetric PCR that efficiently generates single-stranded DNA which can readily be characterized at the end of amplification by hybridization to low-temperature fluorescent probes. We demonstrate here for the first time that monoplex and duplex LATE-PCR amplification and probe target hybridization can be carried out in double layered PDMS microfluidics chips containing dried reagents. Addition of a set of reagents during dry down overcomes the common problem of single-stranded oligonucleotide binding to PDMS. These proof-of-principle results open the way to construction of inexpensive point-of-care devices that take full advantage of the analytical power of assays built using LATE-PCR and low-temperature probes.

Graphical abstract: Construction of a microfluidic chip, using dried-down reagents, for LATE-PCR amplification and detection of single-stranded DNA

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Article information


Submitted
16 Sep 2013
Accepted
25 Sep 2013
First published
25 Sep 2013

Lab Chip, 2013,13, 4635-4641
Article type
Paper

Construction of a microfluidic chip, using dried-down reagents, for LATE-PCR amplification and detection of single-stranded DNA

Y. Jia, P. Mak, C. Massey, R. P. Martins and L. J. Wangh, Lab Chip, 2013, 13, 4635
DOI: 10.1039/C3LC51049B

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