Issue 15, 2013

Immobilisation of hydroxynitrile lyases


Hydroxynitrile lyases are a versatile group of enzymes that are applied both in the laboratory and on an industrial scale. What makes them particularly interesting is that to date five structurally unrelated categories of hydroxynitrile lyases have been discovered. Given their great importance they have often been immobilised utilising many different methodologies. Therefore the hydroxynitrile lyases are ideally suited to compare different immobilisation methods and their dependence on the structural features of the enzyme in question, since the activity is the same in all cases. This review examines all the different immobilisation methods applied to hydroxynitrile lyases and draws conclusions on the effect of the approach.

Graphical abstract: Immobilisation of hydroxynitrile lyases

Article information

Article type
Review Article
30 Nov 2012
First published
29 Jan 2013
This article is Open Access
Creative Commons BY license

Chem. Soc. Rev., 2013,42, 6308-6321

Immobilisation of hydroxynitrile lyases

U. Hanefeld, Chem. Soc. Rev., 2013, 42, 6308 DOI: 10.1039/C3CS35491A

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given.

Read more about how to correctly acknowledge RSC content.

Social activity