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Issue 10, 2013
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Orientation selection in distance measurements between nitroxide spin labels at 94 GHz EPR with variable dual frequency irradiation

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Abstract

Pulsed electron–electron double resonance (PELDOR, also known as DEER) has become a method of choice to measure distances in biomolecules. In this work we show how the performance of the method can be improved at high EPR frequencies (94 GHz) using variable dual frequency irradiation in a dual mode cavity in order to obtain enhanced resolution toward orientation selection. Dipolar evolution traces of a representative RNA duplex and an α-helical peptide were analysed in terms of possible bi-radical structures by considering the inherent ambiguity of symmetry-related solutions.

Graphical abstract: Orientation selection in distance measurements between nitroxide spin labels at 94 GHz EPR with variable dual frequency irradiation

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Publication details

The article was received on 08 Dec 2012, accepted on 15 Jan 2013 and first published on 17 Jan 2013


Article type: Communication
DOI: 10.1039/C3CP44415E
Phys. Chem. Chem. Phys., 2013,15, 3433-3437

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    Orientation selection in distance measurements between nitroxide spin labels at 94 GHz EPR with variable dual frequency irradiation

    I. Tkach, S. Pornsuwan, C. Höbartner, F. Wachowius, S. Th. Sigurdsson, T. Y. Baranova, U. Diederichsen, G. Sicoli and M. Bennati, Phys. Chem. Chem. Phys., 2013, 15, 3433
    DOI: 10.1039/C3CP44415E

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