Issue 57, 2013
From the journal:

Chemical Communications

Mechanical unfolding of long human telomeric RNA (TERRA)†

Miguel Garavís,‡ab   Rebeca Bocanegra,‡c   Elías Herrero-Galán,c   Carlos González,*b   Alfredo Villasante*a  and  J. Ricardo Arias-Gonzalez*de  

* Corresponding authors

a Instituto de Química Física Rocasolano, CSIC, C/Serrano 119, 28006 Madrid, Spain
E-mail: cgonzalez@iqfr.csic.es
Fax: +34 915642431
Tel: +34 915619400

b Centro de Biología Molecular “Severo Ochoa” CSIC-UAM, Universidad Autónoma de Madrid, Nicolás Cabrera 1, 28049 Madrid, Spain
E-mail: avillasante@cbm.uam.es
Fax: +34 911964420
Tel: +34 911964401

c Centro Nacional de Biotecnología (CNB-CSIC), C/Darwin 3, Cantoblanco, 28049 Madrid, Spain

d IMDEA Nanociencia, C/Faraday 9, Cantoblanco, 28049 Madrid, Spain
E-mail: ricardo.arias@imdea.org
Fax: +34 912998725
Tel: +34 912998860

e CNB-CSIC-IMDEA Nanociencia Associated Unit “Unidad de Nanobiotecnología”, Spain

Abstract

We report the first single molecule investigation of TERRA molecules. By using optical-tweezers and other biophysical techniques, we have found that long RNA constructions of up to 25 GGGUUA repeats form higher order structures comprised of single parallel G-quadruplex blocks, which unfold at lower forces than their DNA counterparts.

Graphical abstract: Mechanical unfolding of long human telomeric RNA (TERRA)

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Article information

DOI
https://doi.org/10.1039/C3CC42981D
Article type
Communication
Submitted
22 Apr 2013
Accepted
28 May 2013
First published
29 May 2013

Chem. Commun., 2013,49, 6397-6399

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Mechanical unfolding of long human telomeric RNA (TERRA)

M. Garavís, R. Bocanegra, E. Herrero-Galán, C. González, A. Villasante and J. R. Arias-Gonzalez, Chem. Commun., 2013, 49, 6397 DOI: 10.1039/C3CC42981D

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