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Issue 9, 2013
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Microfluidic free-flow electrophoresis chips with an integrated fluorescent sensor layer for real time pH imaging in isoelectric focusing

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Abstract

Functional microfluidic free-flow electrophoresis chips with integrated fluorescent pH sensors are presented. Polyethylene glycol based structures were fabricated that allowed for integration of both functions on a single microchip. Microchips were applied in free-flow isoelectric focusing of model compounds and proteins with on-line monitoring of pH during microscale electrophoretic separation.

Graphical abstract: Microfluidic free-flow electrophoresis chips with an integrated fluorescent sensor layer for real time pH imaging in isoelectric focusing

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Supplementary files

Article information


Submitted
08 Nov 2012
Accepted
10 Dec 2012
First published
12 Dec 2012

Chem. Commun., 2013,49, 904-906
Article type
Communication

Microfluidic free-flow electrophoresis chips with an integrated fluorescent sensor layer for real time pH imaging in isoelectric focusing

S. Jezierski, D. Belder and S. Nagl, Chem. Commun., 2013, 49, 904
DOI: 10.1039/C2CC38093E

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