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Issue 84, 2013
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Detection of salt bridges to lysines in solution in barnase

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Abstract

We show that salt bridges involving lysines can be detected by deuterium isotope effects on NMR chemical shifts of the sidechain amine. Lys27 in the ribonuclease barnase is salt bridged, and mutation of Arg69 to Lys retains a partially buried salt bridge. The salt bridges are functionally important.

Graphical abstract: Detection of salt bridges to lysines in solution in barnase

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Publication details

The article was received on 23 Jul 2013, accepted on 05 Sep 2013 and first published on 05 Sep 2013


Article type: Communication
DOI: 10.1039/C3CC45602A
Citation: Chem. Commun., 2013,49, 9824-9826

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    Detection of salt bridges to lysines in solution in barnase

    M. P. Williamson, A. M. Hounslow, J. Ford, K. Fowler, M. Hebditch and P. E. Hansen, Chem. Commun., 2013, 49, 9824
    DOI: 10.1039/C3CC45602A

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