Issue 76, 2013
From the journal:

Chemical Communications

In vitro monitoring of the formation of pentamers from the monomer of GST fused HPV 16 L1†

Dong-Dong Zheng,a   Dong Pan,a   Xiao Zha,ab   Yuqing Wu,*a   Chunlai Jiangc  and  Xianghui Yu*c  

* Corresponding authors

a State Key Laboratory of Supramolecular Structure and Materials, Jilin University, Changchun, China
E-mail: yqwu@jlu.edu.cn

b Sichuan Tumor Hospital & Institute, Chengdu, China

c State Engineering Laboratory of AIDS Vaccine, Jilin University, No. 2699, Qianjin Street, Changchun, China
E-mail: xhyu@jlu.edu.cn

Abstract

The recombinant GST fusion protein HPV 16 L1 from E. coli was proved to exist as a monomer rather than a pentamer, providing the possibility of real-time monitoring of the pentamer formation in vitro. Time-dependent kinetic studies of the process were performed for the first time by using static light scattering and western blot analysis, where the essential factors were revealed, offering a new biotechnical approach for virus control and/or the development of anti-viral agents.

Graphical abstract: In vitro monitoring of the formation of pentamers from the monomer of GST fused HPV 16 L1

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Article information

DOI
https://doi.org/10.1039/C3CC44986F
Article type
Communication
Submitted
03 Jul 2013
Accepted
23 Jul 2013
First published
24 Jul 2013

Chem. Commun., 2013,49, 8546-8548

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In vitro monitoring of the formation of pentamers from the monomer of GST fused HPV 16 L1

D. Zheng, D. Pan, X. Zha, Y. Wu, C. Jiang and X. Yu, Chem. Commun., 2013, 49, 8546 DOI: 10.1039/C3CC44986F

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