Issue 66, 2013
From the journal:

Chemical Communications

Universal labeling of 5′-triphosphate RNAs by artificial RNA ligase enzyme with broad substrate specificity†

John C. Haugner IIIa  and  Burckhard Seelig*a  

* Corresponding authors

a Department of Biochemistry, Molecular Biology and Biophysics & BioTechnology Institute, University of Minnesota, 1479 Gortner Ave, Saint Paul, MN 55108, USA
E-mail: seelig@umn.edu
Fax: +1-612-625-5780
Tel: +1-612-626-6281

Abstract

An artificial RNA ligase specific to RNA with a 5′-triphosphate (PPP-RNA) exhibits broad sequence specificity on model substrates and secondary siRNAs with direct applications in the identification of PPP-RNAs through sequencing.

Graphical abstract: Universal labeling of 5′-triphosphate RNAs by artificial RNA ligase enzyme with broad substrate specificity

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Article information

DOI
https://doi.org/10.1039/C3CC44454F
Article type
Communication
Submitted
14 Jun 2013
Accepted
03 Jul 2013
First published
04 Jul 2013

Chem. Commun., 2013,49, 7322-7324

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Universal labeling of 5′-triphosphate RNAs by artificial RNA ligase enzyme with broad substrate specificity

J. C. Haugner III and B. Seelig, Chem. Commun., 2013, 49, 7322 DOI: 10.1039/C3CC44454F

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