Issue 74, 2013
From the journal:

Chemical Communications

A universal real-time PCR assay for rapid quantification of microRNAs via the enhancement of base-stacking hybridization†

Cui-Yuan Yu,a   Bin-Cheng Yin*a  and  Bang-Ce Ye*a  

* Corresponding authors

a Lab of Biosystem and Microanalysis, State Key Laboratory of Bioreactor Engineering, East China University of Science & Technology, Shanghai 200237, P.R. China
E-mail: binchengyin@ecust.edu.cn, bcye@ecust.edu.cn
Fax: +86-21-64252094
Tel: +86-21-64252094

Abstract

Via the base-stacking hybridization strategy, we have developed a universal, one-step real-time quantitative PCR assay for sensitive and selective detection of microRNAs. This proposed assay has several intrinsic features including rapid response, low cost, simple handling procedures, etc.

Graphical abstract: A universal real-time PCR assay for rapid quantification of microRNAs via the enhancement of base-stacking hybridization

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Article information

DOI
https://doi.org/10.1039/C3CC44125C
Article type
Communication
Submitted
01 Jun 2013
Accepted
16 Jul 2013
First published
17 Jul 2013

Chem. Commun., 2013,49, 8247-8249

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A universal real-time PCR assay for rapid quantification of microRNAs via the enhancement of base-stacking hybridization

C. Yu, B. Yin and B. Ye, Chem. Commun., 2013, 49, 8247 DOI: 10.1039/C3CC44125C

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