Issue 65, 2013
From the journal:

Chemical Communications

Fluorescence-activated cell sorting and directed evolution of α-N-acetylgalactosaminidases using a quenched activity-based probe (qABP)†

Kamaladasan Kalidasan,‡a   Ying Su,‡b   Xiaoyuan Wu,‡b   Shao Q. Yao*b  and  Mahesh Uttamchandani*abc  

* Corresponding authors

a Defence Medical and Environmental Research Institute, DSO National Laboratories, 27 Medical Drive, Singapore 117510, Singapore

b Department of Chemistry, National University of Singapore, 3 Science Drive 3, Singapore 117543, Singapore
E-mail: chmyaosq@nus.edu.sg
Fax: +65 6779 1691
Tel: +65 6516 2925

c Department of Biological Science, National University of Singapore, 14 Science Drive 4, Singapore 117543, Singapore
E-mail: mahesh@dso.org.sg
Fax: +65 6485 7033
Tel: +65 6485 7214

Abstract

An α-N-acetylgalactosamine-containing quenched activity-based probe (qABP) was designed and successfully synthesized, and it was subsequently used in directed enzyme evolution experiments aided by fluorescence-activated cell sorting (FACS) for the discovery of an α-N-acetylgalactosaminidase variant with improved catalytic activity.

Graphical abstract: Fluorescence-activated cell sorting and directed evolution of α-N-acetylgalactosaminidases using a quenched activity-based probe (qABP)

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Article information

DOI
https://doi.org/10.1039/C3CC42836B
Article type
Communication
Submitted
17 Apr 2013
Accepted
19 Jun 2013
First published
19 Jun 2013
This article is Open Access
Creative Commons BY license

Chem. Commun., 2013,49, 7237-7239

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Fluorescence-activated cell sorting and directed evolution of α-N-acetylgalactosaminidases using a quenched activity-based probe (qABP)

K. Kalidasan, Y. Su, X. Wu, S. Q. Yao and M. Uttamchandani, Chem. Commun., 2013, 49, 7237 DOI: 10.1039/C3CC42836B

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