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Issue 48, 2013
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A universal flow cytometry assay for screening carbohydrate-active enzymes using glycan microspheres

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Abstract

We describe a simple, multiplexed assay that integrates glycan synthesis, bioconjugation to microspheres, fluorescent chemical/biochemical detection and multiparameter flow cytometric analysis to screen activities of different families of carbohydrate-active enzymes.

Graphical abstract: A universal flow cytometry assay for screening carbohydrate-active enzymes using glycan microspheres

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Supplementary files

Article information


Submitted
21 Dec 2012
Accepted
11 Mar 2013
First published
12 Mar 2013

Chem. Commun., 2013,49, 5441-5443
Article type
Communication

A universal flow cytometry assay for screening carbohydrate-active enzymes using glycan microspheres

A. Chandrasekaran, K. Deng, C. Koh, T. Takasuka, L. F. Bergeman, B. G. Fox, P. D. Adams and A. K. Singh, Chem. Commun., 2013, 49, 5441
DOI: 10.1039/C3CC39155H

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