A novel label-free cascade amplification strategy based on dumbbell probe-mediated rolling circle amplification-responsive G-quadruplex formation for highly sensitive and selective detection of NAD+ or ATP

Abstract

A novel label-free fluorescent sensing scheme for sensitive and selective detection of NAD⁺ and ATP has been developed based on dumbbell probe-mediated rolling circle amplification (D-RCA)-responsive G-quadruplex formation. This approach can detect 0.5 pM for ATP and 100 fM for NAD⁺, much lower than those of previously reported biosensors, and exhibits high discrimination ability.