Issue 20, 2013
From the journal:

Chemical Communications

A new hybrid signal amplification strategy for ultrasensitive electrochemical detection of DNA based on enzyme-assisted target recycling and DNA supersandwich assemblies†

Ying Chen,a   Qiong Wang,a   Jin Xu,a   Yun Xiang,*a   Ruo Yuan*a  and  Yaqin Chaia  

* Corresponding authors

a Key Laboratory on Luminescence and Real-Time Analysis, Ministry of Education, School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, P. R. China
E-mail: yunatswu@swu.edu.cn, yuanruo@swu.edu.cn
Fax: +86-23-68252277
Tel: +86-23-68253172

Abstract

A highly sensitive electrochemical sequence-specific DNA detection strategy is demonstrated by coupling N.BstNB I (a nicking endonuclease)-assisted target recycling amplification with DNA supersandwich assembly signal enhancement. The proposed method avoids any extra chemical labeling steps and offers high selectivity against single-base mismatch sequences and a low detection limit down to 0.36 fM.

Graphical abstract: A new hybrid signal amplification strategy for ultrasensitive electrochemical detection of DNA based on enzyme-assisted target recycling and DNA supersandwich assemblies

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Article information

DOI
https://doi.org/10.1039/C3CC00034F
Article type
Communication
Submitted
02 Jan 2013
Accepted
25 Jan 2013
First published
05 Feb 2013

Chem. Commun., 2013,49, 2052-2054

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A new hybrid signal amplification strategy for ultrasensitive electrochemical detection of DNA based on enzyme-assisted target recycling and DNA supersandwich assemblies

Y. Chen, Q. Wang, J. Xu, Y. Xiang, R. Yuan and Y. Chai, Chem. Commun., 2013, 49, 2052 DOI: 10.1039/C3CC00034F

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