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Issue 1, 2014
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A novel method for monitoring mineralisation in hydrogels at the engineered hard–soft tissue interface

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Abstract

The capacity to study the deposition of mineral within a hydrogel structure is of significant interest to a range of therapies that seek to replace the hard tissues and the hard–soft tissue interface. Here, a method is presented that utilises Confocal Raman microscopy as a tool for monitoring mineralisation within hydrogels. Synthetic hard–soft material interfaces were fabricated by apposing brushite (a sparingly soluble calcium phosphate) and biopolymer gel monoliths. The resulting structures were matured over a period of 28 days in phosphate buffered saline. Confocal Raman microscopy of the interfacial region showed the appearance of calcium phosphate salt deposits away from the original interface within the biopolymeric structures. Furthermore, the appearance of octacalcium phosphate and carbonated hydroxyapatite was observed in the region of the brushite cement opposing the biopolymer gel. This study describes not only a method for analysing these composite structures, but also suggests a method for recapitulating the graduated tissue structures that are often found in vivo.

Graphical abstract: A novel method for monitoring mineralisation in hydrogels at the engineered hard–soft tissue interface

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Supplementary files

Article information


Submitted
22 Apr 2013
Accepted
17 Sep 2013
First published
08 Oct 2013

This article is Open Access

Biomater. Sci., 2014,2, 41-51
Article type
Paper

A novel method for monitoring mineralisation in hydrogels at the engineered hard–soft tissue interface

S. Koburger, A. Bannerman, L. M. Grover, F. A. Müller, J. Bowen and J. Z. Paxton, Biomater. Sci., 2014, 2, 41
DOI: 10.1039/C3BM60102A

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