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Issue 12, 2013
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Molecularly imprinted polymers for the extraction of imiquimod from biological samples using a template analogue strategy

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Abstract

Molecularly Imprinted Polymers (MIPs) against imiquimod, a highly potent immune response modifier used in the treatment of skin cancer, were synthesised using a template analogue strategy and were compared with imprints of the drug itself. An investigation of the complexation between the functional monomer and the template analogue revealed an association constant of 1376 ± 122 M−1, significantly higher than previously reported values for similar systems. The binding characteristics of the synthesised imprinted polymers were evaluated and extremely strong binding for imiquimod was observed while imprinting factors as high as 17 were calculated. When applied as sorbents in solid-phase extraction of imiquimod from aqueous, urine and blood serum samples, clean extracts and recoveries up to 95% were achieved, and it is concluded that while imiquimod imprints exhibited higher capacity for the drug, template analogue imprints are more selective. The results obtained suggest potential applications of imiquimod imprints as sorbents in rapid extraction and monitoring of undesirable systemic release of the drug.

Graphical abstract: Molecularly imprinted polymers for the extraction of imiquimod from biological samples using a template analogue strategy

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Article information


Submitted
08 Feb 2013
Accepted
23 Apr 2013
First published
23 Apr 2013

This article is Open Access

Anal. Methods, 2013,5, 3122-3128
Article type
Paper

Molecularly imprinted polymers for the extraction of imiquimod from biological samples using a template analogue strategy

P. Manesiotis, S. Kashani and P. McLoughlin, Anal. Methods, 2013, 5, 3122
DOI: 10.1039/C3AY40239H

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