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Issue 18, 2013
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Electron ionization-induced release of coded isotopic reporter ions in an m/z zone of minimal interference for quantifiable, multiplexed GC-MS analyses

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Abstract

We describe an isotope coding strategy that enables simultaneous GC-MS analysis of multiple samples for substrate identification and quantification. The method relies on direct measurement of isotopic ethyl carbenium ions serving as mass spectral tags in a zone of minimal interference (ZMI) at m/z 31–37. Sample aldehyde and ketone mixtures were reacted with isotopic 2-aminooxyethyl propionates to illustrate the method, which determined the relative abundance of the mixed compounds with an average 95% accuracy. ZMI reporter ion detection also enables chemoselective substrate profiling and absolute quantification, as demonstrated using a biologically derived sample.

Graphical abstract: Electron ionization-induced release of coded isotopic reporter ions in an m/z zone of minimal interference for quantifiable, multiplexed GC-MS analyses

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Publication details

The article was received on 08 Jul 2013, accepted on 09 Jul 2013 and first published on 25 Jul 2013


Article type: Paper
DOI: 10.1039/C3AY41124A
Citation: Anal. Methods, 2013,5, 4701-4706
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    Electron ionization-induced release of coded isotopic reporter ions in an m/z zone of minimal interference for quantifiable, multiplexed GC-MS analyses

    S. Laulhé, T. E. Geers, X. Shi, X. Zhang and M. H. Nantz, Anal. Methods, 2013, 5, 4701
    DOI: 10.1039/C3AY41124A

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