Issue 23, 2013
From the journal:

Analytical Methods

Imaging cellular uptake and intracellular distribution of TiO2 nanoparticles†

Jichao Zhang,ab   Xiaoqing Cai,*a   Yi Zhang,a   Xiaoming Li,a   Wenxin Li,a   Yangchao Tian,c   Aiguo Li,b   Xiaohan Yu,b   Chunhai Fana  and  Qing Huang*a  

* Corresponding authors

a Laboratory of Physical Biology and Shanghai Synchrotron Radiation Facility, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, People's Republic of China
E-mail: caixiaoqing@sinap.ac.cn, huangqing@sinap.ac.cn
Tel: +86-21-39194068

b Shanghai Synchrotron Radiation Facility, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, People's Republic of China
E-mail: caixiaoqing@sinap.ac.cn, huangqing@sinap.ac.cn
Tel: +86-21-39194068

c National Synchrotron Radiation Laboratory, University of Science and Technology of China, Hefei, Anhui, People's Republic of China

Abstract

Understanding the internalization of nanoparticles in cells is of great interest for diagnostic and therapeutic applications. We investigated the cellular uptake and distribution of TiO2 nanoparticles by high lateral resolution transmission electron microscopy (TEM) and transmission X-ray microscopy. TEM results showed that TiO2 nanoparticle internalization mostly occurred via endocytosis and the nanoparticles were distributed in the cytoplasm. 3D reconstructed tomography also confirmed that TiO2 nanoparticle aggregates were mainly distributed over the cell membrane surface.

Graphical abstract: Imaging cellular uptake and intracellular distribution of TiO2 nanoparticles

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Supplementary files

Article information

DOI
https://doi.org/10.1039/C3AY41121D
Article type
Paper
Submitted
08 Jul 2013
Accepted
09 Sep 2013
First published
10 Sep 2013

Anal. Methods, 2013,5, 6611-6616

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Imaging cellular uptake and intracellular distribution of TiO2 nanoparticles

J. Zhang, X. Cai, Y. Zhang, X. Li, W. Li, Y. Tian, A. Li, X. Yu, C. Fan and Q. Huang, Anal. Methods, 2013, 5, 6611 DOI: 10.1039/C3AY41121D

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