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Issue 19, 2013
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HPLC-DAD-MSn analysis and HPLC quantitation of chemical constituents in the traditional Chinese medicine formula Ya-tong-yi-li-wan

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Abstract

A combination of qualitative and quantitative methods was developed and validated to elucidate the chemical constituents in Ya-tong-yi-li-wan (YTYLW), a traditional Chinese medicine formula used for the treatment of toothache in China. Few reports are available on the chemical analysis of YTYLW, so far. For qualitative analysis, a method based on high-performance liquid chromatography coupled with diode-array detection and electrospray ionization tandem mass spectrometry (HPLC/DAD/ESI-MSn) was established. A total of 48 compounds in YTYLW were characterized, including 10 bufadienolides, 9 isoflavones, 7 chalcones, 7 saponins, 6 flavanones, 4 flavonols, 2 flavones, 2 coumarins, and 1 pterocarpan. For quantitative analysis, 12 major compounds in YTYLW were simultaneously determined by HPLC-UV at 270 nm in 9 batches of commercial products. Precision, reproducibility and accuracy of the method were fully validated. The method established in this study could be used for the quality control of YTYLW.

Graphical abstract: HPLC-DAD-MSn analysis and HPLC quantitation of chemical constituents in the traditional Chinese medicine formula Ya-tong-yi-li-wan

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Article information


Submitted
26 Jun 2013
Accepted
27 Jul 2013
First published
31 Jul 2013

Anal. Methods, 2013,5, 5241-5247
Article type
Paper

HPLC-DAD-MSn analysis and HPLC quantitation of chemical constituents in the traditional Chinese medicine formula Ya-tong-yi-li-wan

K. Liu, X. Qiao, Q. Wang, W. Song, D. Guo and M. Ye, Anal. Methods, 2013, 5, 5241
DOI: 10.1039/C3AY41054D

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