Jump to main content
Jump to site search

Issue 14, 2013
Previous Article Next Article

Double-strand DNA-templated synthesis of copper nanoclusters as novel fluorescence probe for label-free detection of biothiols

Author affiliations

Abstract

In this work, we report a simple and sensitive method for the detection of biothiols including glutathione (GSH), cysteine (Cys) and homocysteine (Hcy), using fluorescent copper nanoclusters synthesized by a double-stranded DNA template (DNA-CuNCs) as a probe. The photoluminescence intensity of DNA-CuNCs was found to be quenched effectively with the increase in the concentration of biothiols due to the formation of nonfluorescent coordination complexes between the DNA-CuNCs and biothiols, whereas the fluorescence of DNA-CuNCs was not changed in the presence of other amino acids at a 10-fold higher concentration. Satisfactory detection limits and linear relationships for the detection of GSH, Cys and Hcy were obtained The resulting calibration plots exhibited good linear correlations in the range from 2.0 × 10−6 to 1.0 × 10−4 mol L−1 for Cys, 2.0 × 10−6 to 8.0 × 10−5 mol L−1 for GSH, and 5.0 × 10−6 to 2.0 × 10−4 mol L−1 for Hcy. The detection limits of Cys, GSH and Hcy were 2 μmol L−1, 2 μmol L−1 and 5 μmol L−1, respectively. In addition, the method was successfully applied in the detection of biothiols in human plasma samples.

Graphical abstract: Double-strand DNA-templated synthesis of copper nanoclusters as novel fluorescence probe for label-free detection of biothiols

Back to tab navigation

Publication details

The article was received on 16 Jan 2013, accepted on 06 May 2013 and first published on 08 May 2013


Article type: Paper
DOI: 10.1039/C3AY40088C
Anal. Methods, 2013,5, 3577-3581

  •   Request permissions

    Double-strand DNA-templated synthesis of copper nanoclusters as novel fluorescence probe for label-free detection of biothiols

    Y. Hu, Y. Wu, T. Chen, X. Chu and R. Yu, Anal. Methods, 2013, 5, 3577
    DOI: 10.1039/C3AY40088C

Search articles by author

Spotlight

Advertisements