Issue 11, 2013

Separation and identification of phosphorothioate oligonucleotides by HILIC-ESIMS

Abstract

A combined hydrophilic interaction chromatography (HILIC) electrospray ionization mass spectrometry (ESI-MS) approach for the separation and identification of phosphorothioate oligonucleotides is described. Phosphorothioate 21-mer and 23-mer were separated by HILIC and detected using selected ion monitoring (SIM) ESI-MS. Phosphorothioates could be detected from 50 nM solutions suggesting effectiveness comparable to ion pairing reversed phase chromatography approaches.

Graphical abstract: Separation and identification of phosphorothioate oligonucleotides by HILIC-ESIMS

Supplementary files

Article information

Article type
Communication
Submitted
08 Dec 2012
Accepted
25 Apr 2013
First published
26 Apr 2013

Anal. Methods, 2013,5, 2657-2659

Separation and identification of phosphorothioate oligonucleotides by HILIC-ESIMS

R. Easter, C. Barry, J. Caruso and P. Limbach, Anal. Methods, 2013, 5, 2657 DOI: 10.1039/C3AY26519F

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