Electrochemical behavior of luteolin and its detection based on macroporous carbon modified glassy carbon electrode

Abstract

In this paper, a simple and highly sensitive electrochemical sensor based on a macroporous carbon modified glassy carbon electrode (MPC/GCE) was developed and used for detecting luteolin in phosphate buffer solution (PBS, pH 7.0) for the first time. Cyclic voltammetry (CV) and differential pulse voltammetric methods (DPV) were applied to investigate the electrochemical behavior and mechanism of luteolin detection on the MPC/GCE. The results indicated that MPC/GCE can greatly enhance the electrocatalytic activity towards the redox of luteolin. A series of experimental parameters including the pH of the supporting electrolyte, accumulation potential and time were optimized. In neutral solution, the MPC/GCE showed a low detection limit of 1.3 × 10⁻⁹ M (S/N = 3), a favorable ability of anti-interference to electroactive molecules and good reproducibility and long-term stability. Finally, the method was successfully applied for the determination of luteolin in a capsule of Lamiophlomis rotata Kudo (Chinese patent medicine) with satisfying results.