Preparation of a novel skeleton weak anion-exchange monolith by single electron transfer-living radical polymerization for protein separation in high performance liquid chromatography

Abstract

A novel skeleton weak anion-exchange monolith was prepared by single electron transfer-living radical polymerization (SET-LRP) for high performance liquid chromatography (HPLC). In this study, vinyl ester resin was used as the monomer, ethyleneglycol dimethacrylate as the cross linking agent, carbon tetrachloride as the initiator, ferrous powder as the catalyst, and N,N,N′,N′-tetramethylethylenediamine as the ligand to supply amine-groups. The polymerization conditions were optimized. The characters of the monolith were investigated by scanning electron microscopy, Fourier transform infrared spectroscopy and mercury porosimeter, respectively. Good skeleton structure properties were obtained. Moreover, the prepared monolith was used as HPLC stationary phase to separate immune globulin G (IgG) from human plasma with high efficiency. The influences of buffer concentration and pH of the mobile phase on the separation of IgG were investigated. In addition, the monolith was also used to separate the mixture of beef serum albumin (BSA), IgG and lysozyme (Lys) successfully.