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Issue 6, 2014
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A handheld magnetic sensing platform for antigen and nucleic acid detection

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Abstract

The core requirements for point-of-care (POC) diagnostics necessitate low-cost, portability, easily integrated sample preparation, and quick measurement time. Frequency-shift based magnetic sensing is a measurement technique utilizing a complementary metal-oxide-semiconductor (CMOS) integrated-circuit (IC) chip for magnetic label detection. The sensing scheme leverages the low-cost manufacturing of IC chips while demonstrating the potential for multiplexing capabilities. In this article, we present modifications to this scheme for POC viability. We introduce a handheld reusable reader and a disposable open-well cartridge for the detection of nucleic acids and antigens. The diagnostic system utilizes a novel “magnetic freezing” technique to reduce measurement time, obviates baseline measurement before or during biological assay, and reduces sensor noise. We utilize these enhancements for the room temperature, amplification-free detection of a 31 base-pair DNA oligomer and the interferon-γ (IFN-γ) protein. We have demonstrated reliable measurements down to 100 pM for the DNA assay and 1 pM for the protein.

Graphical abstract: A handheld magnetic sensing platform for antigen and nucleic acid detection

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Publication details

The article was received on 16 Oct 2013, accepted on 10 Dec 2013 and first published on 10 Dec 2013


Article type: Paper
DOI: 10.1039/C3AN01947K
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Analyst, 2014,139, 1403-1411
  • Open access: Creative Commons BY-NC license
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    A handheld magnetic sensing platform for antigen and nucleic acid detection

    A. Pai, A. Khachaturian, S. Chapman, A. Hu, H. Wang and A. Hajimiri, Analyst, 2014, 139, 1403
    DOI: 10.1039/C3AN01947K

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