Issue 23, 2013

Whole organ cross-section chemical imaging using label-free mega-mosaic FTIR microscopy

Abstract

FTIR chemical imaging has been demonstrated as a promising technique to construct automated systems to complement histopathological evaluation of biomedical tissue samples. The rapid chemical imaging of large areas of tissue has previously been a limiting factor in this application. Consequently, smaller areas of tissue have previously had to be sampled, possibly introducing sampling bias and potentially missing diagnostically important areas. In this report a high spatial resolution chemical image of a whole prostate cross section is shown comprising 66 million pixels. Each pixel represents an area 5.5 × 5.5 μm2 of tissue and contains a full infrared spectrum providing a chemical fingerprint. The data acquisition time was 14 hours, thus showing that a clinical time frame of hours rather than days has been achieved.

Graphical abstract: Whole organ cross-section chemical imaging using label-free mega-mosaic FTIR microscopy

Supplementary files

Article information

Article type
Communication
Submitted
04 Sep 2013
Accepted
02 Oct 2013
First published
02 Oct 2013
This article is Open Access
Creative Commons BY license

Analyst, 2013,138, 7066-7069

Whole organ cross-section chemical imaging using label-free mega-mosaic FTIR microscopy

P. Bassan, A. Sachdeva, J. H. Shanks, M. D. Brown, N. W. Clarke and P. Gardner, Analyst, 2013, 138, 7066 DOI: 10.1039/C3AN01674A

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements