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Issue 15, 2013
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Sensitivity of activatable reactive oxygen species probes by fluorescence spectroelectrochemistry

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Abstract

We have developed a new analytical method of evaluating activatable fluorescent probes for ROS detection using integrated fluorescence spectroelectrochemistry. The Tafel formalism was applied to describe the process of the probes' oxidation under electrochemical conditions and identify a novel parameter defined as the threshold oxidation potential. This potential can serve as an approximation to the equilibrium potential and can be utilized for determining the sensitivity of a probe to oxidation. Based upon the measured values of threshold potentials, the order of sensitivity towards oxidation among several commonly used probes was determined to be the following (from highest to lowest): 2,7-dihydrodichlorofluorescein > dihydroethidium > dihydrorhodamine 123 > dihydrorhodamine 6G. The presented approach opens up a new direction in synthesizing and screening novel ROS probes with a well-defined sensitivity for in vitro and in vivo applications.

Graphical abstract: Sensitivity of activatable reactive oxygen species probes by fluorescence spectroelectrochemistry

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Publication details

The article was received on 07 Mar 2013, accepted on 04 May 2013 and first published on 08 May 2013


Article type: Paper
DOI: 10.1039/C3AN00459G
Analyst, 2013,138, 4363-4369

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    Sensitivity of activatable reactive oxygen species probes by fluorescence spectroelectrochemistry

    S. T. Wang, N. G. Zhegalova, T. P. Gustafson, A. Zhou, J. Sher, S. Achilefu, O. Y. Berezin and M. Y. Berezin, Analyst, 2013, 138, 4363
    DOI: 10.1039/C3AN00459G

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