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Issue 6, 2013
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Antibody orientation on biosensor surfaces: a minireview

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Abstract

Detection elements play a key role in analyte recognition in biosensors. Therefore, detection elements with high analyte specificity and binding strength are required. While antibodies (Abs) have been increasingly used as detection elements in biosensors, a key challenge remains – the immobilization on the biosensor surface. This minireview highlights recent approaches to immobilize and study Abs on surfaces. We first introduce Ab species used as detection elements, and discuss techniques recently used to elucidate Ab orientation by determination of layer thickness or surface topology. Then, several immobilization methods will be presented: non-covalent and covalent surface attachment, yielding oriented or random coupled Abs. Finally, protein modification methods applicable for oriented Ab immobilization are reviewed with an eye to future application.

Graphical abstract: Antibody orientation on biosensor surfaces: a minireview

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Publication details

The article was received on 01 Dec 2012, accepted on 06 Jan 2013 and first published on 22 Jan 2013


Article type: Minireview
DOI: 10.1039/C2AN36787D
Citation: Analyst, 2013,138, 1619-1627
  • Open access: Creative Commons BY license
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    Antibody orientation on biosensor surfaces: a minireview

    A. K. Trilling, J. Beekwilder and H. Zuilhof, Analyst, 2013, 138, 1619
    DOI: 10.1039/C2AN36787D

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