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Issue 3, 2013
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Rapid photochemical surface patterning of proteins in thiol–ene based microfluidic devices

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Abstract

The suitable optical properties of thiol–ene polymers combined with the ease of modifying their surface for the attachment of recognition molecules make them ideal candidates in many biochip applications. This paper reports the rapid one-step photochemical surface patterning of biomolecules in microfluidic thiol–ene chips. This work focuses on thiol–ene substrates featuring an excess of thiol groups at their surface. The thiol–ene stoichiometric composition can be varied to precisely control the number of surface thiol groups available for surface modification up to an average surface density of 136 ± 17 SH nm−2. Biotin alkyne was patterned directly inside thiol–ene microchannels prior to conjugation with fluorescently labelled streptavidin. The surface bound conjugates were detected by evanescent wave-induced fluorescence (EWIF), demonstrating the success of the grafting procedure and its potential for biochip applications.

Graphical abstract: Rapid photochemical surface patterning of proteins in thiol–ene based microfluidic devices

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Publication details

The article was received on 03 Oct 2012, accepted on 20 Nov 2012 and first published on 20 Nov 2012


Article type: Paper
DOI: 10.1039/C2AN36424G
Citation: Analyst, 2013,138, 845-849
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    Rapid photochemical surface patterning of proteins in thiol–ene based microfluidic devices

    J. P. Lafleur, R. Kwapiszewski, T. G. Jensen and J. P. Kutter, Analyst, 2013, 138, 845
    DOI: 10.1039/C2AN36424G

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